Figure 2. DCs infected with nrMVA stimulate OVA presentation to T cells.

DCs were infected for 6 h with nrMVA at a MOI of 0.05, 0.5 or 5 in the presence of OVA protein (10 µg/ml). Non-stimulated and OVA pulsed DCs were used as controls. (A) After infection, DCs were cultured with either naïve CD8⁺ T cells from OT-I mice (left panel) or naïve CD4⁺ T cells from OT-II mice (right panel) at different ratios for 5 days. Cells were pulsed with [³H]-thymidine 16 h before harvesting. Proliferation of T cells was assessed by measuring [³H]-thymidine incorporation. (B) MVA-infected DCs were cultured with CFSE labeled naïve T cells of OT-I (upper panel) or OT-II mice (lower panel) for 5 days. Proliferation was assessed by flow cytometry. p<0.01 (**); p<0.001 (***).