Figure 4. IL-4-, IL-2- and IFN-γ-producing cells were stimulated using nrMVA as adjuvant.

Splenocytes of immunized mice were re-stimulated in vitro for 48 h with the OVA protein to determine the numbers of (A) IL-4- and (B) IL-2-secreting cells. (C) Cells were also stimulated for 16 h with the dominant MHC class I restricted peptide SIINFEKL to determine the numbers of IFN-γ secreting CD8⁺ T cells. Results are presented as specific spot forming units (SFU)/1×10⁶ cells. The SEM of quadruplicate values is indicated by vertical lines. The values reported are those obtained from stimulated cells subtracted of background from non-stimulated cells. Values of non-stimulated cells were lower than 50 SFU/1×10⁶ cells. The results were statistically significant at p<0.05 (*); p<0.0001 (***).