Fig. 6.

scn8aa is widely expressed within the CNS and PNS and motor nerves develop normally in nav mutants. (A) Expression of scn8aa in a 24 hpf embryo by the posterior lateral line ganglion (arrowheads) and RB neurons. Scale bar, 200 μm. (B) enlarged image of region indicated in the top panel showing presumptive RB neurons (arrows highlight a few) expressing scn8aa. Scale bar, 50 μm. (C) Expression of scn8aa in a 48 hpf embryo is more widespread. Asterisk denotes the trigeminal ganglion which is shown at higher power in (D). Boxed area highlights RBs shown in (E). (D) Enlarged image of region containing the trigeminal ganglion that was denoted by an asterisk in (C). (E) Presumptive RBs (right, arrows highlight a few) expressing scn8aa. (F) Sideview of the mid-trunk focused on the dorsal branches of the motor nerves (arrows) in a wild type sibling. Motor nerves were labeled with MAb Zn5 at 66 hpf. (G) Sideview of the mid-trunk showing normal dorsal branches of the motor nerves (arrows) in a nav mutant at 66 hpf. Anterior is to the left and dorsal up in both panels.