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. 2010 Jul 8;5(7):e11472. doi: 10.1371/journal.pone.0011472

Figure 4. Loss of myelin in the optic nerve of rotenone-treated rats and its protection by rAAV5-NDI1.

Figure 4

Sagittal sections of the optic nerve were obtained from the rats treated with rotenone for 8 weeks and were stained for myelin basic protein (MBP) using specific antibody. A: representative pictures of the MBP staining intensity. Scale bar  = 100 µm. B: histograms comparing the fluorescence intensity of MBP staining. MBP staining intensity was evaluated by ImageJ (NIH) with 8 measures per animal and 4 animals per group. **p<0.01, n.s. (no-significant) Student's T-test. Error bars represent the mean ± SD. For A and B: a, control; b, rats treated with rotenone for 8 weeks; c, rAAV5-NDI1 injected immediately after the rotenone microspheres.