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Calculation of DNA repair capacity. Primary fibroblasts, keratinocytes and melanocytes were transfected either with a mixture of irradiated DsRedexpress and pEGFP-N1 or with a mixture of unirrdiated DsRedexpress and pEGFP-N1. FACS analysis was carried out 36 h post transfection. The negative control serves as reference for data interpretation and to correct the results for potential variations in mixing ratio and point of time for FACS analysis.
