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Dorsal rhizotomy reduces GFP and SP labeling in spinal cord. GFP- (A and B) and substance P-ir (C and D) in rat lumbar spinal cord. Unilateral dorsal rhizotomy was performed 6 weeks after i.v. mannitol pretreatment and i.t. rAAV5-GFP injection. GFP-ir is reduced ipsilaterally to dorsal rhizotomy (A and B). Reduction in SP-ir in the same section (C and D) demonstrates the efficacy of the surgical procedure.
