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. 2010 Jun 16;30(24):8096–8101. doi: 10.1523/JNEUROSCI.0036-10.2010

Figure 1.

Figure 1.

OEA stimulates c-fos mRNA expression in oxytocin neurons of the hypothalamus. A, B, Rat brain coronal sections showing the effects of vehicle (A) or OEA (10 mg kg−1, i.p.) (B) on c-fos mRNA levels in the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus. Calibration bar, 1 mm. C, D, Time course (in minutes) of OEA-induced c-fos mRNA expression in PVN (C) and SON (D). Open bar, vehicle; closed bar, OEA (10 mg kg−1, i.p.). E, F, In situ double hybridization images showing the effects of vehicle (E) or OEA (F) on mRNA levels of c-fos (white spots) and oxytocin (dark spots) in the PVN. Calibration bar, 0.25 mm. G, Immunohistochemical detection of c-fos protein (red) and oxytocin (green) in neurons of the PVN. Calibration bar, 50 μm. **p < 0.01, ***p < 0.001, versus respective control; n = 5–6.