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. 2010 May 29;11:343. doi: 10.1186/1471-2164-11-343

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Copyright ©2010 Markou et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Differential recruitment of cardiomyocyte RNAs to polysomes.Cardiomyocytes were serum-starved (20 h). Polysomes prepared by sucrose density centrifugation. Total and polysomal RNA were extracted and analysed using microarrays. A, A₂₅₄profiles for sucrose density centrifugation. B, Heatmap of the mean raw fluorescence values for the 6425 probesets with significantly different expression in polysomal and total RNA pools in unstimulated cells [Log₂scale; 7 (cyan) through 10 (black) to 13 (red)]. C, Functional classification of top 10^thpercentile transcripts detected in cardiomyocytes. D, Heatmap for the 515 probesets of the top 10^thpercentile with significantly different expression (FDR < 0.05, >1.5-fold difference) in polysomal and total RNA pools in unstimulated cells [Log₂scale; 9.5 (cyan) through 11.5 (black) to 13.5 (red)].