Table 2.
Primers used for qPCR.
| Gene | Accession no. | Size (bp) | Forward primer | Reverse primer |
|---|---|---|---|---|
| Klf2 | NM_001007684 | 96 | CACACAGGTGAGAAGCCTTATCAT (874-897) | CCGTGTGCTTGCGGTAGTG (952-970) |
| Klf6 | NM_031642 | 89 | GCTCCCACTTGAAAGCACATC (641-661) | TTCTTGCAAAACGCCACTCA (710-729) |
| Klf10 | NM_031135 | 149 | CCATGAGCTGCGACTGGAA (183-201) | TAAGGTGGAGTCAAACAGAATGCT (307-331) |
| Klf11 | NM_001037354 | 85 | CCTGATCTACCAAAGGACTTCCA (311-333) | CTCATGGAGCCAACAGGGA (377-395) |
| Klf15 | NM_053536 | 65 | TGCGGCTGGAGGTTTTCA (1347-1364) | TTCACACCCGAGTGAGATCGT (1391-1411) |
| Klf16 | NM_001127604 | 79 | TCACACCTGCGGACTCACA (516-534) | CGGAACGGGCGAACTTCT (577-594) |
| Rps3 | NM_001009239 | 94 | AAAGTGGCCACAAGAGGTCTGT (280-301) | CATAGCAGGCCCTTCGAACT (354-373) |
| Rps6 | NM_017160 | 82 | AAGGTAAGAAGCCCAGGACCAA (509-530) | ATACGTCGGCGTTTGTGTTG (571-590) |
mRNA sequences (accession numbers provided) for established genes were obtained from the Rat Genome Database (http://rgd.mcw.edu, viewed at http://www.ncbi.nlm.nih.gov/entrez). Nucleotide positions are shown in parentheses for each primer.