Table 2. Slope coefficients and R² values for the comparison of one gap distances calculated for SINA-aligned sequences extracted from different regions within the 16S rRNA gene sequence to one gap distances calculated using sequences aligned by different methods.

Region	Statistic	RDP	greengenes	MUSCLE	Needleman
V19	Slope	1.06	1.17	1.04	0.93
	R2	0.97	0.77	0.98	0.99
V12	Slope	1.13	1.25	1.11	0.93
	R2	0.80	0.52	0.77	0.91
V13	Slope	1.08	1.20	1.07	0.93
	R2	0.88	0.62	0.92	0.93
V14	Slope	1.06	1.16	1.05	0.94
	R2	0.94	0.74	0.96	0.97
V2	Slope	1.04	1.21	1.16	0.97
	R2	0.94	0.67	0.64	0.95
V23	Slope	1.04	1.18	1.09	0.95
	R2	0.96	0.74	0.92	0.96
V3	Slope	1.00	1.11	2.07	1.02
	R2	0.91	0.67	0.14	0.96
V35	Slope	1.01	1.12	1.04	0.95
	R2	0.98	0.83	0.97	0.98
V4	Slope	1.00	1.09	1.08	0.98
	R2	0.99	0.77	0.87	0.98
V6	Slope	1.09	1.42	3.04	0.98
	R2	0.66	0.14	0.30	0.97
V69	Slope	1.07	1.21	1.10	0.94
	R2	0.92	0.70	0.91	0.97
V89	Slope	1.05	1.18	1.12	0.96
	R2	0.94	0.78	0.86	0.98
V9	Slope	1.08	1.19	1.58	0.96
	R2	0.80	0.67	0.36	0.96