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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2010 May 31;49(3):392–400. doi: 10.1016/j.freeradbiomed.2010.04.028

Table 1.

Incubation of MCF-10F cells with E2-3,4-Q alone or in the presence of NAcCys1

E2-3,4-Q alone E2-3,4-Q: NAcCys (1:0.5) E23,4-Q: NAcCys (1:1)
Detected Compounds2 1 10 30 1 10 30 1 10 30
pmol/10 million cells
4-OHE1(E2) 4.05
± 0.32		 7.79
± 0.34		 14.2
± 1.41		 2.96
± 0.24		 5.79
± 0.51		 9.57
± 1.15		 1.17
± 0.26		 3.07
± 0.32		 5.11
± 0.47
4-OCH3E1(E2) 49.7
± 4.27		 437
± 50.9		 824
± 120		 41.9*
± 4.06		 329*
± 62.3		 593*
± 59.6		 32.3*
± 5.48		 196*
± 28.9		 302*
± 51.3
4-OHE1(E2)-2-SG 0.58
± 0.05		 3.13
± 0.38		 6.39
± 0.77		 0.65
± 0.06		 3.75
± 0.59		 8.10
± 1.54		 0.74*
± 0.06		 4.36*
± 0.31		 10.2*
± 1.71
4-OHE1(E2)-2-Cys 3.95
± 0.71		 8.78
± 0.56		 28.1
± 2.40		 4.42
± 0.46		 10.6*
± 0.72		 34.7*
± 2.49		 5.02
± 0.70		 12.2*
± 1.28		 42.1*
± 2.84
4-OHE1(E2)-2-NAcCys 1.36
± 0.28		 6.37
± 0.81		 15.9
± 2.82		 1.63
± 0.29		 8.15*
± 1.04		 21.6*
± 1.61		 1.98*
± 0.26		 10.3*
± 1.53		 28.0*
± 3.41
4-OHE1(E2)-1-N3Ade 0.39
± 0.03		 2.22
± 0.24		 5.42
± 0.60		 0.34*
± 0.01		 1.70*
± 0.24		 3.76*
± 0.26		 0.28*
± 0.02		 1.12*
± 0.14		 1.67*
± 0.35
4-OHE1(E2)-1-N7Gua 0.36
± 0.02		 2.08
± 0.18		 4.97
± 0.44		 0.30
± 0.04		 1.52
± 0.39		 3.50*
± 0.40		 0.26*
± 0.03		 0.99*
± 0.13		 1.55*
± 0.19
1

MCF-10F cells were incubated at 37 °C for 24 h with E2-3,4-Q (1, 10 or 30 µM) alone or in the presence of different ratios of NAcCys.

2

The compounds were identified and quantified by HPLC-ECD and the adducts were confirmed by UPLC-MS/MS; values are an average of three replicates from three independent pools of medium each from a set of five flasks; ± standard deviation.

*

The value is significantly different from that in the cell s not treated with NAcCys (p<0.05) as determined by the t-test.