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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2010 May 31;49(3):392–400. doi: 10.1016/j.freeradbiomed.2010.04.028

Table 2.

Incubation of E6 cells with E2-3,4-Q alone or in the presence of NAcCys1

E2-3,4-Q alone E2-3,4-Q: NAcCys (1:0.5) E2-3,4-Q: NAcCys (1:1)
Detected Compounds2 1 10 30 1 10 30 1 10 30
pmol/10 million cells
4-OHE1(E2) 0.41
± 0.07		 1.34
± 0.16		 2.35
± 0.30		 0.27
± 0.06		 0.90
± 0.05		 1.50
± 0.18		 0.14
± 0.03		 0.65
± 0.06		 0.92
± 0.09
4-OCH3E1(E2) 60.2
± 5.45		 555
± 29.4		 1142
± 74.8		 48.9*
± 5.89		 414*
± 29.9		 761*
± 85.7		 37.5*
± 2.47		 264*
± 65.8		 345*
± 81.3
4-OHE1(E2)-2-SG 0.76
± 0.05		 6.38
± 1.0		 14.9
± 2.36		 0.86*
± 0.06		 7.58
± 2.26		 18.5*
± 1.83		 1.0*
± 0.09		 8.98*
± 1.48		 21.8*
± 2.41
4-OHE1(E2)-2-Cys 4.52
± 0.48		 11.4
± 0.95		 32.7
± 3.43		 5.18
± 0.48		 14.1*
± 1.09		 41.0*
± 3.23		 6.27*
± 0.67		 16.8*
± 1.35		 48.2*
± 3.17
4-OHE1(E2)-2-NAcCys 1.80
± 0.37		 8.53
± 0.96		 18.6
± 3.26		 2.30
± 0.46		 11.6
± 2.74		 28.5*
± 3.28		 2.85*
± 0.40		 15.2*
± 2.29		 42.6*
± 2.08
4-OHE1(E2)-1-N3Ade 0.49
± 0.06		 2.92
± 0.27		 6.22
± 0.59		 0.41
± 0.04		 2.36
± 0.58		 4.37*
± 0.32		 0.32*
± 0.03		 1.58*
± 0.16		 1.86*
± 0.35
4-OHE1(E2)-1-N7Gua 0.47
± 0.08		 2.76
± 0.15		 5.82
± 0.54		 0.39
± 0.02		 2.07*
± 0.36		 3.94*
± 0.74		 0.33*
± 0.02		 1.42*
± 0.25		 1.79*
± 0.21
1

E6 cells were incubated at 37 °C for 24 h with E2-3,4-Q (1, 10, or 30 µM) alone or in the presence of different ratios of NAcCys.

2

The compounds were identified and quantified by HPLC-ECD and the adducts were confirmed by UPLC-MS/MS; values are an average of three replicates from three independent pools of medium each from a set of five flasks; ± standard deviation.

*

The value is significantly different from th at in the cell s not treated with NAcCys (p<0.05) as determined by the t-test.