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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2010 May 31;49(3):392–400. doi: 10.1016/j.freeradbiomed.2010.04.028

Table 3.

Incubation of MCF-10F cells with 4-OHE2 alone or in the presence of NAcCys1

4-OHE2 alone 4-OHE2: NAcCys (1:0.5) 4-OHE2: NAcCys (1:1)
Detected Compounds2 1 10 30 1 10 30 1 10 30
pmol/10 million cells
4-OHE1(E2) 2.20
± 0.45		 9.49
± 2.04		 29.5
± 3.45		 1.11
± 0.33		 5.47
± 0.71		 14.2
± 2.17		 0.27
± 0.07		 2.42
± 0.45		 7.37
± 1.4
4-OCH3E1(E2) 1139
± 106		 3788
± 353		 10648
± 1211		 1282
± 124		 4500*
± 259		 14944*
± 1316		 1461*
± 200		 5598*
± 500		 23736*
± 1467
4-OHE1(E2)-2-SG 1.10
± 0.21		 7.48
± 0.77		 12.2
± 3.34		 1.19
± 0.16		 8.57
± 0.57		 14.4
± 2.09		 1.32*
± 0.15		 9.87
± 2.19		 17.3
± 2.94
4-OHE1(E2)-2-Cys 5.16
± 0.55		 9.98
± 0.24		 37.3
± 1.26		 5.74
± 0.53		 11.7*
± 0.72		 46.2*
± 4.29		 6.76*
± 1.08		 14.2*
± 2.29		 58.6*
± 3.30
4-OHE1(E2)-2-NAcCys 5.0
± 0.65		 11.5
± 2.0		 31.0
± 3.86		 5.57
± 1.10		 13.2
± 2.26		 38.6*
± 3.95		 6.84*
± 0.60		 16.6*
± 3.31		 51.2*
± 3.63
4-OHE1(E2)-1-N3Ade 0.84
± 0.09		 3.76
± 0.53		 11.2
± 2.38		 0.68*
± 0.07		 2.63*
± 0.20		 6.07*
± 2.20		 0.53*
± 0.03		 1.14*
± 0.19		 1.57*
± 0.34
4-OHE1(E2)-1-N7Gua 0.70
± 0.19		 2.86
± 0.28		 9.99
± 1.20		 0.59
± 0.05		 2.11*
± 0.28		 6.26*
± 1.24		 0.51*
± 0.04		 1.05*
± 0.08		 1.52*
± 0.29
1

MCF-10F cells were incubated at 37 °C for 24 h with 4-OHE2 (1, 10 or 30 µM) alone or in the presence of different ratios of NAcCys.

2

The compounds were identified and quantified by HPLC-ECD and the adducts were confirmed by UPLC-MS/MS; values are an average of three replicates from three independent pools of medium each from a set of five flasks; ± standard deviation.

*

The value is significantly different from th at in the cells not treated with NAcCys (p<0.05) as determined by the t-test.