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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2010 May 31;49(3):392–400. doi: 10.1016/j.freeradbiomed.2010.04.028

Table 4.

Incubation of E6 cells with 4 -OHE2 alone or in presence of NAcCys1

4-OHE2 alone 4-OHE2: NAcCys (1:0.5) 4-OHE2: NAcCys (1:1)
Detected Compounds2 1 10 30 1 10 30 1 10 30
pmol/10 million cells
4-OHE1(E2) 1.29
± 0.11		 4.46
± 1.01		 20.1
± 1.76		 0.78
± 0.09		 2.86
± 0.86		 10.5
± 2.40		 0.21
± 0.07		 0.59
± 0.10		 3.56
± 1.13
4-OCH3E1(E2) 2077
± 327		 4669
± 440		 15112
± 1652		 2377
± 338		 5718*
± 342		 19804*
± 1325		 2785*
± 335		 7599*
± 963		 36372*
± 3355
4-OHE1(E2)-2-SG 2.38
± 0.47		 13.9
± 2.02		 33.0
± 4.19		 2.67
± 0.55		 16.4
± 0.57		 40.8*
± 1.70		 3.07
± 0.70		 18.6*
± 2.32		 50.4*
± 3.23
4-OHE1(E2)-2-Cys 6.19
± 1.29		 18.12
± 2.53		 38.5
± 7.06		 7.25
± 1.16		 22.3*
± 2.27		 50.4
± 7.53		 8.80
± 1.81		 28.4*
± 2.92		 64.2*
± 7.61
4-OHE1(E2)-2-NAcCys 6.22
± 0.66		 17.9
± 1.53		 40.5
± 6.46		 7.23
± 0.96		 22.1*
± 1.92		 54.3*
± 6.82		 8.78*
± 1.07		 26.8*
± 2.04		 70.8*
± 11.9
4-OHE1(E2)-1-N3Ade 1.33
± 0.28		 4.18
± 0.87		 41.9
± 4.97		 1.07
± 0.05		 2.81*
± 0.33		 25.3*
± 1.88		 0.53*
± 0.03		 1.63*
± 0.26		 4.00*
± 0.73
4-OHE1(E2)-1-N7Gua 0.97
± 0.11		 3.97
± 0.59		 39.6
± 4.65		 0.79*
± 0.09		 2.70*
± 0.51		 24.3*
± 1.48		 0.51*
± 0.04		 1.58*
± 0.25		 4.59*
± 0.83
1

E6 cells were incubated at 37 °C for 24 h with 4-OHE2 (1, 10 or 30 µM) alone or in the presence of different ratios of NAcCys.

2

The compounds were identified and quantified by HPLC-ECD and the adducts were confirmed by UPLC-MS/MS; values are an average of three replicates from three independent pools of medium each from a set of five flasks; ± standard deviation.

*

The value is significantly different from th at in the cell s not treated with NAcCys (p<0.05) as determined by the t-test.