Table 5.
Incubation of MCF-10F cells with 10 µM 4-OHE2 alone or in the presence of NAcCys1
| Treatment ratio: 4-OHE2: NAcCys | |||||
|---|---|---|---|---|---|
| Detected Compounds 2 | 1:0 | 1:0.1 | 1:0.3 | 1:1 | 1:3 |
| pmol/10 million cells | |||||
| 4-OHE1(E2) | 11.5 ± 2.73 |
9.38 ± 2.35 |
5.73 ± 0.92 |
3.03 ± 0.59 |
0.70 ± 0.09 |
| 4-OCH3E1(E2) | 3684 ± 346 |
3931 ± 427 |
4515 ± 275 |
5780* ± 386 |
8143* ± 942 |
| 4-OHE1(E2)-2-SG | 8.35 ± 0.75 |
8.52 ± 0.92 |
9.24 ± 1.12 |
10.4* ± 0.95 |
12.2* ± 1.78 |
| 4-OHE1(E2)-2-Cys | 11.9 ± 1.71 |
12.2 ± 1.06 |
14.3 ± 1.77 |
17.2* ± 1.96 |
20.8* ± 3.99 |
| 4-OHE1(E2)-2-NAcCys | 13.7 ± 2.07 |
14.2 ± 1.72 |
16.7 ± 1.72 |
20.2* ± 1.30 |
25.1* ± 2.30 |
| 4-OHE1(E2)-1-N3Ade | 4.43 ± 0.84 |
4.31 ± 0.45 |
3.23 ± 0.25 |
1.29* ± 0.45 |
nd3 |
| 4-OHE1(E2)-1-N7Gua | 3.56 ± 0.72 |
3.43 ± 0.51 |
2.62 ± 0.39 |
1.13* ± 0.27 |
nd |
1
MCF-10F cells were incubated at 37 °C for 24 h with 4-OHE2 (10 µM) alone or in the presence of different ratios of NAcCys.
2
The compounds were identified and quantified by HPLC-ECD and the adducts were confirmed by UPLC-MS/MS; values are an average of three replicates from three independent pools o f medium each from a set of five flasks; ± standard deviation.
3
not detected.
*
The value is significantly different from th at in the cells not treated with NAcCys (p<0.05) as determined by the t-test.