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. Author manuscript; available in PMC: 2011 Jun 18.
Published in final edited form as: Biochem Biophys Res Commun. 2010 May 20;397(1):117–119. doi: 10.1016/j.bbrc.2010.05.081

Fig. 2. “Reverse” Far Western blot probed with labeled N-terminus of human Topo I.

Fig. 2

The blot was prepared by SDS PAGE of 1 µg of unlabeled phosphorylated or non-phosphorylated GST-CTD fusion protein per lane, followed by blotting to nitrocellulose [8]. The protein segment comprising amino acids 1–199 of human Topo I was labeled by 32P with casein kinase II and used as a probe. Lanes 1 and 2: GST-CTD fusion protein phosphorylated by CTDK-I; lane 3: non-phosphorylated GST-CTD fusion protein.