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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Mol Microbiol. 2010 May 4;76(5):1111–1126. doi: 10.1111/j.1365-2958.2010.07192.x

Figure 2.

Figure 2

Chaperone activity of DegP is required for efficient BFP expression in E2348/69. A) bfpA expression by the strains, ALN188 and E2348/69 after 45 minutes was determined by qPCR of cDNA isolated from the cultures. ΔCt values were obtained by subtracting the Ct of the bfpA amplicon from that of the 16S RNA amplicon. The graph represents the mean of three independent experiments. Error bars indicate the standard deviations from the means. B) Temporal expression of bundlin by the EPEC strains, E2348/69, ALN188, and ALN188, complemented in trans with either DegPS210A (pCAdegPS210A) or wild-type DegP (pCAdegP), was monitored by immunoblot analysis of the bundlin and MBP proteins. The data presented in the graph represent % bundlin immunoblot band density, relative to MBP immunoblot density for each lane of the blot. Each point in the graph represents the mean result from three independent immunoblot experiments. The graph represents the cumulative results from the three experiments, and shows mean bundlin immunoblot band density, relative to MBP band density for each lane of the blots. The error bars represent standard deviations from the means.