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. Author manuscript; available in PMC: 2011 Jun 1.

Published in final edited form as: Mol Microbiol. 2010 May 4;76(5):1111–1126. doi: 10.1111/j.1365-2958.2010.07192.x

Effect of 90 μM LacNac equivalents of EP-II-189 on BFP retraction. A) Autoaggregation index of E2348/69 following 30 minutes incubation in the presence of 90 μM LacNAc equivalents of LacNAc-BSA, EP-II-189 or 0.8 mg/mL underivatized BSA. Data are presented at two time points, 30 min and 3 h, and represent the mean of three experiments. **p < 0.0001 relative to organisms exposed to underivatized BSA. B) Bundlin expression, determined by western immunoblot analysis, in E2348/69 in the presence of 90 μM relative LacNAc concentration of EP-II-189, LacNAc-BSA or equivalent concentrations of underivatized polyacrylamide (PAA) or BSA. Graph bars represent the bundlin band density relative to that of the MBP density, for each lane of the blot. Data were normalized using the underivatized BSA negative control, and represent the means of three experiments. **p < 0.0001 relative to organisms exposed to underivatized BSA. C) Effect of EP-II-189 on bfpA expression, as assessed by real-time PCR of bfpA and 16s RNA from cDNA isolated from the bacteria following a 30 min or 1 h incubation with or without EP-II-189. Fold gene up-regulation was determined by calculating 2^−ΔΔCt, as described in the materials and methods section. **p < 0.0001, *p < 0.01 relative to organisms exposed to underivatized BSA. D) Effect of a 30 minute incubation with 90 μM LacNAc equivalent of EP-II-189 on light production by E2348/69 harbouring the plasmids pRMHlux1-5 (LEE 1-5 promoters) and pRMHlux (a promoterless plasmid). Bars represent the mean of three experiments. For all panels, error bars are the standard deviations from the means.

Effect of 90 μM LacNac equivalents of EP-II-189 on BFP retraction. A) Autoaggregation index of E2348/69 following 30 minutes incubation in the presence of 90 μM LacNAc equivalents of LacNAc-BSA, EP-II-189 or 0.8 mg/mL underivatized BSA. Data are presented at two time points, 30 min and 3 h, and represent the mean of three experiments. **p < 0.0001 relative to organisms exposed to underivatized BSA. B) Bundlin expression, determined by western immunoblot analysis, in E2348/69 in the presence of 90 μM relative LacNAc concentration of EP-II-189, LacNAc-BSA or equivalent concentrations of underivatized polyacrylamide (PAA) or BSA. Graph bars represent the bundlin band density relative to that of the MBP density, for each lane of the blot. Data were normalized using the underivatized BSA negative control, and represent the means of three experiments. **p < 0.0001 relative to organisms exposed to underivatized BSA. C) Effect of EP-II-189 on bfpA expression, as assessed by real-time PCR of bfpA and 16s RNA from cDNA isolated from the bacteria following a 30 min or 1 h incubation with or without EP-II-189. Fold gene up-regulation was determined by calculating 2^−ΔΔCt, as described in the materials and methods section. **p < 0.0001, *p < 0.01 relative to organisms exposed to underivatized BSA. D) Effect of a 30 minute incubation with 90 μM LacNAc equivalent of EP-II-189 on light production by E2348/69 harbouring the plasmids pRMHlux1-5 (LEE 1-5 promoters) and pRMHlux (a promoterless plasmid). Bars represent the mean of three experiments. For all panels, error bars are the standard deviations from the means.