FIGURE 4. Effect of IGF-1 on the expression of phosphorylated Akt in SV and IMA SMCs.
Cultured SMCs were incubated with IGF-1 (100 ng/ml) for 0.5, 2, 4, 12 and 24 hrs. Protein concentration was determined in the cell lysate and each well was loaded with 20 µg of protein. Each bar represents the ratio of pPKB/PKB (mean ±SEM) from five independent experiments. p <0.05 vs control.