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. Author manuscript; available in PMC: 2011 Jun 25.
Published in final edited form as: Biochem Biophys Res Commun. 2010 May 20;397(2):245–250. doi: 10.1016/j.bbrc.2010.05.092

Figure 2. Immunoprecipitations of Flag-55K and Flag-42K CDK9 proteins for proteomic analysis.

Figure 2

A. Plasmid expression vectors for Flag-tagged 55K and 42K CDK9 proteins were transfected into HeLa cells and immunoprecipitations with the Flag antibody were performed at Day 2 post-transfection. An immunoblot was performed to measure the expression levels of the Flag-tagged proteins and the relative amount of Cyclin T1 that co-immunoprecipitated with each protein. B. The plasmid vectors were transfected into HeLa cells and immunoprecipitations with the Flag antibody were performed. A portion of the immunoprecipitate was analyzed on a SDS-polyacrylamide gel which was stained with Colloidal Blue. The remaining portion was subjected to a proteomics analysis to identify proteins present in the precipitations.