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. Author manuscript; available in PMC: 2011 Jun 25.
Published in final edited form as: Biochem Biophys Res Commun. 2010 May 20;397(2):245–250. doi: 10.1016/j.bbrc.2010.05.092

Figure 3. Ku70 specifically associates with the 55K CDK9 protein.

Figure 3

A. Plasmid expression vectors for Flag-tagged 55K and 42K CDK9 proteins and parental Flag vector were transfected into HeLa cells and immunoprecipitations were performed with the Flag antibody (left panel) or a Ku70 antiserum (right panel). Proteins present in immunoprecipitations were examined by immunoblotting. B. Cell extracts were prepared from HeLa cells and immunoprecipitations were performed with antisera against CDK9, Ku70, and CDK7 as indicated. The presence of the indicated proteins in immunoprecipitations was examined in immunoblots; HeLa cell lysate is shown on right lane. C. A HeLa cell extract was treated with and without DNase as indicated, and immunoprecipitations were performed with a CDK9 antiserum. The indicated proteins in the immunoprecipitates were examined in an immunoblot.