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Illustration of the 0.8% agarose gel used to purify the large (2065 bp) dsDNA fragment of pBS II K (+) following digestion with XhoI and AlwNI. After electrophoresis, lanes A, C, and E are excised from the gel and stained with ethidium bromide (dashed black lines). These lanes contain two bands (white bands). Using these bands as markers, the area that contains the 2065 bp dsDNA fragment is cut out of lanes B and D (dashed red boxes), and then purified by electroelution.
