. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: Anal Biochem. 2010 May 4;404(1):40–44. doi: 10.1016/j.ab.2010.04.035

Table 2.

Calculated amounts of labeled methylglucosamine using MRM with and without correction factor (CF)

Methylglucosamine (Glc-MA)^a	True amount nmol	Calculated amount (without CF) nmol	Calculated amount (with CF) nmol
Glc-1-¹³C-MA	0.200	0.243 ± 0.006	0.198 ± 0.005
Glc-2-¹³C-MA	0.200	0.219 ± 0.002	0.200 ± 0.002
Glc-1,2-¹³C-MA	0.200	0.258 ± 0.002	0.202 ± 0.002
Glc-D₂-MA	0.200

^aThe quantification of labeled methylglucosamine was performed by multiple reaction monitoring scans (MRM) using Glc-D₂-MA as internal standard. The values are presented as mean ± standard error of three independent samples.