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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Cell Neurosci. 2010 May 12;44(4):362–373. doi: 10.1016/j.mcn.2010.05.001

Figure 2. Faint DsRed-fluorescent cells express markers of granule cells.

Figure 2

(A), fluorimetric quantification of intensity of red fluorescence reveals two subpopulations of DsRed cells in hGFAP-DsRed mice. (B,C) Confocal Z-stacks and projections illustrating bright (arrowheads) and faint (arrows) DsRed fluorescence in the EGL and IGL. (D–I) faint red fluorescence is visible in cells displaying Reelin immunoreactivity at both P2 and P4 (D–I, arrows), whereas bright DsRed fluorescent cells do not display Reelin immunostaining (arrowheads). (J–O) faint red fluorescence is also visible in Zic 1/2 positive cells (J–M, arrows). In contrast, cells with bright red fluorescence are negative for Zic1/2 (J–O, arrowhead). All images are 1 µm thick single confocal optical section; side panels show Z-stack projections.