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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Cell Neurosci. 2010 May 12;44(4):362–373. doi: 10.1016/j.mcn.2010.05.001

Figure 4. Characterization of EGFP reporter expression and DsRed fluorescence in CGE;CAG-EGFP; hGFAP-DsRed mice.

Figure 4

Triple transgenic mice were analyzed at P4 after tamoxifen injections at P1–P3. (A–D), arrows: EGFP+ cells in the EGL express strong (A–B) or weak (C–D) DsRed fluorescence. Blue is DAPI. (E–G), closed arrow: an EGFP+ cell in the EGL that expresses Pax6 immunoreactivity (blue); open arrow: faint DsRed fluorescent cells that expresses weak Pax6 immunoreactivity; (H–M), closed arrow: an EGFP+/DsRed fluorescent cell in the EGL that is Pax6-negative; open arrow: a faint DsRed fluorescent cell in the EGL that expresses weak Pax6 immunoreactivity; (N–P), open arrows: DsRed fluorescent cells in the IGL/Purkinje cell layer that express weak or strong Pax6 immunoreactivity; closed arrowhead: strong DsRed fluorescent cell in the Purkinje cell layer (presumably a Bergmann glial cell) that is Pax6-negative. All images are 1 µm thick single confocal optical sections; side panels show Z-stack analyses.