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A. Western blotting of CA II in GIST882 cells. A positive 30 kDa polypeptide of CA II was observed in the cultured cells. Recombinant human CA II was used as a positive control (the first lane). NRS = normal rabbit serum. B. In Western blotting of primary tumors, CA II was expressed strongly in most GISTs, irrespective of KIT or PDGFRA mutation type. Phosphoinositide-3- kinase (PI3-K) stain was a loading control.
