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. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: Oncogene. 2009 Aug 31;28(45):4022–4033. doi: 10.1038/onc.2009.253

Figure 4.

Figure 4

Induction of epithelial to mesenchymal transition (EMT) at the tumor–host border. Tumor tissues were collected 21 days after co-transplantation of MIM-R hepatocytes alone (R) or in combination with M-HT myofibroblasts (R+M-HT). Serial sections of tumor tissues were used to display both tumor architecture (T,t) and skin (epidermis) morphology (S,s) by standard histology (trichrome and hematoxylin/eosin (H&E) staining), and immunohistochemical staining was performed to visualize EMT markers. Tumor sections were stained with anti-green fluorescent protein antibody to distinguish between murine skin (S,s) and exogenous, subcutaneous MIM-R hepatocytes (T,t). Dashed lines depict tumor-host borders. In the tumor cells, loss of the epithelial markers E-cadherin and total β-catenin from the plasma membrane and the accumulation of nuclear β-catenin (ABC) indicate an EMT at the tumor–host border. Insets show tumor (t) and skin (s) sections at higher magnification. Murine skin serves as internal control for staining. Scale bar, 100 μm. Dashed lines indicate tumor–host borders.