Figure 5.

Formation of stable FAs within filopodial actin. Keratinocytes were grown for 24 h and subsequently double-transfected with GFP-α-actinin and DsRed-vinculin. EGF stimulated cells were analyzed in phase contrast and fluorescence over time. Time series illustrate the assembly of stable FAs along actin bundles originated from filopodia and cross-linked via α-actinin. White arrowheads show co-localization of stable FAs (vinculin) and actin bundles (α-actinin) during elongation. Time points are given in seconds. Scale bar = 5 µm. Slightly affected image quality is caused by the Avalanche photo diode used and a thereby reduced resolution for time lapse analyses.