Figure 6.

Myosin localization and assembly in filopodial actin. (A) Keratinocytes were fixed and stained for myosin II (green) and actin (red). The overview image is additionally overlaid with a DIC-image. The box indicates the zoomed area shown on the right side. Here, white arrowheads highlight the localization of myosin II in actin bundles emerging from filopodia. (B) Double-transfection with YFP-MLC and DsRed-α-actinin was performed on motile keratinocytes. Cells were analyzed in fluorescence and phase contrast over time. The overview image in phase contrast was recorded slightly after time series acquisition. Myosin could be identified within α-actinin containing actin bundles originated from filopodia. White arrowheads point to regions where α-actinin is partially exchanged against myosin. Time points are given in seconds. Scale bars = 10 µm.