Figure 7.

Effective connection of stable FAs with filopodial actin fibers. Keratinocytes were grown for 24 h and transfected with GFP-actin. Regions of filopodial actin were bleached to visualize retrograde flow within filopodia with (A) and without (B) FAs conjunction. White boxes in the overview images indicate zoomed areas shown in the time series. Red boxes illustrate bleached regions. Bleaching was performed at time point 10 s. Red lines highlight the progression of bleached regions in the GFP-images. For a better visualization GFP-images were background subtracted in the time series. Time points are given in seconds. Scale bars = 10 µm. (C) Averaged retrograde flow rates for filopodial actin bundles with (light gray, n = 18, out of 9 cells) and without (dark gray, n = 15, out of 6 cells) connection to FAs. Asterisks indicate a significant difference between both mean values (p value < 0.001). Error bars display the standard error (s.e.).