Fig. 3.

XylE transcriptional fusion analyses of the inhibitory action of UpaZ on PSE locus transcription. (A) Schematic of the PSE regions cloned into the promoterless xylE reporter plasmid. Each clone was amplified with the same forward primer (F) located just inside the upstream IR and contains the promoter in the on orientation and extending 44 bp (clone 8), 135 bp (clone 10), and 229 bp (clone 11) downstream of the transcriptional start site. (B) Northern blot analysis of xylE transcription when each clone is placed in the mpiM44 or mpiM44ΔupaZ background.