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. 2010 Jun 1;107(26):11841–11846. doi: 10.1073/pnas.1003953107

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Fig. 4. — Biogenesis blockade of both prepachytene and perinatal piRNAs in Mov10l1^−/− testes. (A) MILI is unloaded in P10 and P14 Mov10l1^−/− testes. One tenth of the immunoprecipitated material was used for detection of associated RNAs, whereas the remaining was used for Western blotting (WB) to detect MILI. (B) MILI immunoprecipitations with P10 Mov10l1^−/−, Mov10l1^+/−, and serial dilutions (1:2) of P10 wild-type testicular extracts. (C) Profile of read lengths in total small RNA (18–32 nt) libraries from P10 Mov10l1^+/− and Mov10l1^−/− testes. (D) MIWI2 is localized to the cytoplasm in Mov10l1^−/− perinatal (P0) gonocytes (arrows). (E) MILI and MIWI2 are devoid of piRNAs in Mov10l1^−/− newborn (P0) testes.