Fig. 2.

Rapid myelin clearance in JHD mice is restored by endogenous antibodies. (A and B), JHD and WT mice underwent bone marrow transplantation (BMT) with WT whole bone marrow. Sciatic nerve crush was performed 7 wk following BMT. (A) Western blot of serum from WT and JHD mice collected before and after BMT, probed with anti-IgG, anti-IgM, and anti-albumin antibodies. (10 μg total protein per lane) (B) Western blot of sciatic nerve lysates from WT and JHD mice at 10 d postcrush probed with anti-MBP, anti-P₀, and anti-β-actin antibodies (500 ng total protein per lane). (C) Western blot of sciatic nerve lysate samples from JHD mice probed with MBP and albumin antibodies. Samples were taken from uncrushed nerves, crushed JHD nerves, and nerves harvested from crushed JHD mice after passive transfer of serum, purified IgM and IgG, anti-P₀, anti-GFP, or anti-firefly luciferase antibody (1 μg total protein per lane). (D) Average level of MBP protein relative to JHD control nerve at 10 d following injury in JHD mice that received passive transfer of serum, purified IgM or IgG, or monoclonal antibody [via i.p. injection at 2 and 6 d postcrush (DPC)]. All data are from male mice and are presented as mean ± SEM, n = 8–9 animals (*P < 0.05; **P < 0.01).