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. 2010 Jun 14;107(26):11918–11923. doi: 10.1073/pnas.1001749107

Fig. 2.

Fig. 2.

dCD14+/dNK cell interaction induces Tregs. (A) T cells cocultured with the indicated cell combinations were analyzed (at day 7) for the surface expression of CD25, CD127, and CD49d and for the intracellular expression of FOXP3. Two- or three-color immunofluorescence analyses were performed to evaluate cell proliferation and expression of the indicated markers. A shows a representative experiment of six performed. (B) The expression of FOXP3 on proliferating T cells was evaluated in 10 different experiments (i.e., by using pNK, dNK, and dCD14+ cells derived from 10 different donors). Bars indicate the percentage mean (±SEM) of proliferating FOXP3+ T cells assessed in the indicated coculture conditions (n.s., not significant). (C) T cells cocultured with dCD14+/dNK cells were analyzed (at day 7) for the expression of CD39, GITR, and CTLA-4. Two- or three-color immunofluorescence analyses were performed to evaluate the expression of the indicated markers. C shows a representative experiment of three performed. (D) T cells were cocultured as indicated for 35 d and analyzed for the intracellular expression of FOXP3. D shows a representative experiment of three performed.