Fig. 5.

IDO mRNA induction in dCD14⁺ cells following treatment with IFN-γ or transwell coculture with dCD14⁺/dNK cells. (A) IDO mRNA expression was analyzed in freshly isolated dCD14⁺ cells or in dCD14⁺ cells incubated for 14 h with the indicated IFN-γ concentrations. (B) Freshly isolated dCD14⁺ cells that had been conditioned in transwell cocultures with dCD14⁺/dNK cells (Fig. 4A) or that were further cultured alone for 24 h were analyzed for IDO mRNA expression. RT–PCR was performed with primers specific for IDO and for β-actin as positive control. PCR products were run on a 0.8% agarose gel and visualized by ethidium bromide staining. Similar results were obtained in five independent experiments.