Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Photoactivatable FP as Molecular Highlighters
PA-GFP is activated 100-fold by a brief intense pulse of 400 nm light to a restricted ROI. This allows the FP-labeled protein to be selectively activated in the ROI. The measurement of fluorescence intensity in and away from the ROI with time after photoactivation defines, respectively, the immobile fraction of the FP-labeled protein and the kinetics of the mobile protein.
