Table 2.
Groups, treatments, and results of the radioimmunoassay, gas liquid chromatography (for plasma methadone) and solution hybridization RNase TCA precipitation assay. The values represent the means and SEM. mRNA measured in attomole/μ g total RNA. The values omitted from darkened rows are represented graphically in appropriate figure. N/A = not assayed.
| Group | VVV | VMV | CMC | CVC | CVV | VVC | |
|---|---|---|---|---|---|---|---|
| Pavlovian conditioning | Vehicle | Cocaine | Cocaine | Vehicle | |||
| Osmotic mini pumps | Vehicle | Methadone | Methadone | Vehicle | Vehicle | ||
| Prime II | Vehicle | Cocaine | Vehicle | Cocaine | |||
| Plasma Methadone (ng/ml) | N/A | 451 (14) | 491 (10) | N/A | N/A | N/A | |
| Plasma | ACTH (pg/ml) | 461 (56) | 419 (52) | 434 (56) | 382 (49) | 467 (43) | 450 (52) |
| CORT (ng/ml) | 343 (56) | 394 (65) | 416 (122) | 238 (34) | 300 (51) | 462 (96) | |
|
Lateral Hypothalamus (LH) Medial Hypothalamus (MH) |
Hcrt mRNA | Figure 2 | |||||
| POMC mRNA | 15.2 (1.7) | 16.0 (1.8) | 16.3 (2.2) | 15.7 (2.5) | 17.0 (0.9) | 14.2 (2.1) | |
| MOP-r mRNA | 0.20 (0.02) | 0.20 (0.02) | 0.20 (0.03) | 0.18 (0.03) | 0.20 (0.02) | 0.19 (0.03) | |
| Anterior Pituitary (AP) | POMC mRNA | 410 (18) | 430 (25) | 383 (14) | 425 (18) | 394 (28) | 391 (29) |
| Nucleus Accumbens Core (NAcC) | POMC mRNA | 1.23 (0.15) | 1.23 (0.18) | 1.35 (0.14) | 0.94 (0.18) | 1.25 (0.07) | 1.87 (0.53) |
| ppEnk mRNA | 59.0 (9.2) | 62.6 (7.5) | 64.5 (7.3) | 73.6 (11.4) | 62.8 (4.7) | 67.0 (11.0) | |
| MOP-r mRNA | Figure 3, Panel A | ||||||
| Nucleus Accumbens Shell (NAcS) | MOP-r mRNA | 0.40 (0.03) | 0.41 (0.06) | 0.41 (0.06) | 0.42 (0.03) | 0.42 (0.02) | 0.44 (0.04) |
| Caudate-Putamen (CP) | ppDyn mRNA | Figure 4 | |||||
| MOP-r mRNA | 0.12 (0.01) | 0.13 (0.01) | 0.13 (0.01) | 0.18 (0.03) | 0.13 (0.01) | 0.14 (0.02) | |
| DR2 mRNA | Figure 5 | ||||||
| Basolateral Amygdala (BLA) | MOP-r mRNA | Figure 3, Panel B | |||||
| Central Amygdala (CE) | ppDyn mRNA | 6.31 (0.63) | 5.21 (1.17) | 7.14 (0.42) | 6.57 (0.52) | 7.02 (1.03) | 5.38 (0.57) |
| MOP-r mRNA | 1.54 (0.10) | 1.50 (0.09) | 1.47 (0.14) | 1.50 (0.13) | 1.50 (0.06) | 1.49 (0.08) | |
| Ventral Tegmental Area (VTA) | MOP-r mRNA | 0.37 (0.02) | 0.40 (0.07) | 0.42 (0.04) | 0.39 (0.02) | 0.38 (0.01) | 0.40 (0.03) |
| Frontal Cortex (FC) | ppDyn mRNA | 0.89 (0.07) | 0.96 (0.07) | 0.98 (0.06) | 1.01 (0.07) | 0.91 (0.06) | 0.89 (0.15) |
| ppEnk mRNA | 32.5 (3.6) | 31.7 (6.0) | 35.9 (5.1) | 24.9 (1.2) | 30.7 (2.7) | 31.8 (2.4) | |
| MOP-r mRNA | Figure 3, Panel C | ||||||