Table 2.
Group | VVV | VMV | CMC | CVC | CVV | VVC | |
---|---|---|---|---|---|---|---|
Pavlovian conditioning | Vehicle | Cocaine | Cocaine | Vehicle | |||
Osmotic mini pumps | Vehicle | Methadone | Methadone | Vehicle | Vehicle | ||
Prime II | Vehicle | Cocaine | Vehicle | Cocaine | |||
Plasma Methadone (ng/ml) | N/A | 451 (14) | 491 (10) | N/A | N/A | N/A | |
Plasma | ACTH (pg/ml) | 461 (56) | 419 (52) | 434 (56) | 382 (49) | 467 (43) | 450 (52) |
CORT (ng/ml) | 343 (56) | 394 (65) | 416 (122) | 238 (34) | 300 (51) | 462 (96) | |
Lateral Hypothalamus (LH) Medial Hypothalamus (MH) |
Hcrt mRNA | Figure 2 | |||||
POMC mRNA | 15.2 (1.7) | 16.0 (1.8) | 16.3 (2.2) | 15.7 (2.5) | 17.0 (0.9) | 14.2 (2.1) | |
MOP-r mRNA | 0.20 (0.02) | 0.20 (0.02) | 0.20 (0.03) | 0.18 (0.03) | 0.20 (0.02) | 0.19 (0.03) | |
Anterior Pituitary (AP) | POMC mRNA | 410 (18) | 430 (25) | 383 (14) | 425 (18) | 394 (28) | 391 (29) |
Nucleus Accumbens Core (NAcC) | POMC mRNA | 1.23 (0.15) | 1.23 (0.18) | 1.35 (0.14) | 0.94 (0.18) | 1.25 (0.07) | 1.87 (0.53) |
ppEnk mRNA | 59.0 (9.2) | 62.6 (7.5) | 64.5 (7.3) | 73.6 (11.4) | 62.8 (4.7) | 67.0 (11.0) | |
MOP-r mRNA | Figure 3, Panel A | ||||||
Nucleus Accumbens Shell (NAcS) | MOP-r mRNA | 0.40 (0.03) | 0.41 (0.06) | 0.41 (0.06) | 0.42 (0.03) | 0.42 (0.02) | 0.44 (0.04) |
Caudate-Putamen (CP) | ppDyn mRNA | Figure 4 | |||||
MOP-r mRNA | 0.12 (0.01) | 0.13 (0.01) | 0.13 (0.01) | 0.18 (0.03) | 0.13 (0.01) | 0.14 (0.02) | |
DR2 mRNA | Figure 5 | ||||||
Basolateral Amygdala (BLA) | MOP-r mRNA | Figure 3, Panel B | |||||
Central Amygdala (CE) | ppDyn mRNA | 6.31 (0.63) | 5.21 (1.17) | 7.14 (0.42) | 6.57 (0.52) | 7.02 (1.03) | 5.38 (0.57) |
MOP-r mRNA | 1.54 (0.10) | 1.50 (0.09) | 1.47 (0.14) | 1.50 (0.13) | 1.50 (0.06) | 1.49 (0.08) | |
Ventral Tegmental Area (VTA) | MOP-r mRNA | 0.37 (0.02) | 0.40 (0.07) | 0.42 (0.04) | 0.39 (0.02) | 0.38 (0.01) | 0.40 (0.03) |
Frontal Cortex (FC) | ppDyn mRNA | 0.89 (0.07) | 0.96 (0.07) | 0.98 (0.06) | 1.01 (0.07) | 0.91 (0.06) | 0.89 (0.15) |
ppEnk mRNA | 32.5 (3.6) | 31.7 (6.0) | 35.9 (5.1) | 24.9 (1.2) | 30.7 (2.7) | 31.8 (2.4) | |
MOP-r mRNA | Figure 3, Panel C |