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. Author manuscript; available in PMC: 2010 Jul 9.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Aug 26;375(4):522–525. doi: 10.1016/j.bbrc.2008.08.082

Fig. 2.

Fig. 2

Phi29 DNA polymerase specifically amplifies B. burgdorferi B31circular plasmids. B. burgdorferi B31 genomic DNA with human genomic DNA was amplified with Phi29 DNA polymerase using primers specific for circular plasmids cp26 and cp32-6, and linear plasmids lp28-3 and lp36. The amount of specific plasmid amplified was monitored by real-time PCR using plasmid specific gene primers (BBB08 for plasmid cp26, BBM04 for plasmid cp32-6, BBH05/06 for plasmid lp28-3 and BBK21 for plasmid lp36). Samples without addition of Phi29 DNA polymerase were used as a negative control.