Table 2.
Primers used in Real-time PCR
| Primer name | Sequence | Comment |
|---|---|---|
| 5′BBB08 | ATGAAAAAAAAGTTTAATTTTATTTTTC | Amplifies ORF BBB08 on |
| 3′BBB08 | ATTGTAAAAATTTTTCAATTGC | circular plasmid cp26 |
| 5′BBB18 | ATGCTAAGGATCCATGAAGTATAC | Amplifies ORF BBB18 on |
| 3′BBB18 | ATTATTCCCATTCTATGG | circular plasmid cp26 |
| 5′BBA03 | ATGAAAAAAACGATTATTG | Amplifies ORF BBA03 on linear |
| 3′BBA03 | GCTATATAGTGTCTTTAAG | plasmid lp54 |
| 5′BBA24 | ATAATGTTATGATTAAAT | Amplifies ORF BBA24 on linear |
| 3′BBA24 | TTAGTTATTTTTGCATTTTTC | plasmid lp54 |
| 5′BBM04 | ATGGCTTTAAAAGGC | Amplifies ORF BBM04 on |
| 3′BBM04 | TTAACCACCAGCTTC | circular plasmid cp32-6 |
| 5′BBH05 | ACTGGGGCACTATTTGG | Amplifies ORF BBH05/06 on |
| 3′BBH06 | AGATTTTAGCAGGGGAG | linear plasmid lp28-3 |
| 5′BBK21 | ATGGATCAAAAAAAACC | Amplifies ORF BBK21 on linear |
| 3′BBK21 | ATTTACAGTATATTTTTTAAAGCGC | plasmid lp36 |