Fig. 5.

Steric hindrance of lymphocyte surface labeling by trans-sialidase. Thymocytes were transferred with Neu5Az with increased amounts of trans-sialidase (30–600 ng/10⁶ cells). The first lane corresponds to a cell lysate that was treated with the lowest amount of enzyme. Left and right panels are two exposures of the same blot. Note that although other surface markers were transferred with Neu5Az at high enzyme amounts, CD45 remains to be the major acceptor even when the cells were disrupted prior to labeling.