Table I.
Molecules identified by MALDI-TOF
| Access | Protein | MW empiric | MW in silico |
|---|---|---|---|
| AAB59613 | Integrin-α 4 | +AH4-150 | 116 |
| NP_002196 | Integrin-α 5 | +AH4-150 | 116 |
| NP_690621 | PTK7 | +AH4-150 | 114 |
| AAC31672 | Integrin-α L | +AH4-150 | 136 |
| NP_690621 | PTK7 | +AH4-135 | 114 |
| P08195 | CD98 | +AH4-120 | 58 |
| NP_031381 | HSP90AB1 | +AH4-90 | 84 |
| NP_006073 | α-Tubulin | +AH4-60 | 50 |
| AAH08633 | β-Actin | +AH4-42 | 42 |
Human Jurkat CD45−/− cells were treated with trans-sialidase and Neu5Azα2-3LacßOMe. Transferred azido-sialic acid was tagged with Phos-FLAG and acceptor molecules were pull-down with anti-FLAG mAb after lysis with Triton X-100, solved by SDS–PAGE and protein bands processed for MALDI-TOF assay.