Table 2. Chemical Shifts of H2′ Protons in Loop vs Stem^a.

sequence	avg measured δ of H2′ in WC pair not terminal or 5′ of AG or GA loop	measured δ of H2′ 5′ of AG or GA loop	NUCHEMICS prediction of δ of H2′ 5′ of AG or GA loop
5′-GACGAGCGUCA	4.41 ± 0.13	3.97	4.28 ± 0.14
ACUGCGAGCAG-5′
5′-GGCAGGCCb	4.40 ± 0.14	4.06	4.64
CCGGACGG-3′
5′-GACUAGAGUCA	4.41 ± 0.18	4.16	4.57 ± 0.02
ACUGAGAUCAG-5′
5′-GACAAGUGUCA	4.44 ± 0.14	4.15	4.63 ± 0.05
ACUGUGAACAG-5′
5′-GCGGACGCc	4.36 ± 0.11	3.74	4.44
CGCAGGCG-5′
5′-GGUAGGCCA	4.43 ± 0.15	3.78	4.02 ± 0.10
ACCGGAUGG-5′
5′-GGiCGAiGCCAd	4.37 ± 0.11	4.15	N/A
ACCiGAGiCGG-5′
avg	4.41 ± 0.14	4.00 ± 0.17	4.43 ± 0.22

^aComparison of H2′ proton chemical shifts (δ in ppm) in residues 5′ of tandem AG or GA imino pairs with H2′ shifts of all other nonterminal Watson−Crick (WC) paired residues and with NUCHEMICS prediction of chemical shift.

^bReference (23).

^cReference (22).

^dReference (51).