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Albumin effects ROS production and caspase-12 activity in mRPTs ex vivo. mRPTs from male wt or CAT-Tg mice were incubated in 5 mM d-glucose serum-free DMEM in the absence or presence of albumin (0, 30, or 60 μg/ml) for 16 hours. (A) ROS generation was assessed after 10 minutes of incubation in Krebs buffer and expressed as relative light units (RLU). (B) Caspase-12 activity assays were performed after 16 hours of incubation in mRPTs.
