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. 2010 Jun;21(6):943–954. doi: 10.1681/ASN.2009030242

Figure 9.

Figure 9.

Albumin affects caspase-3 activity and apoptosis in HK-2 cells in vitro. HK-2 cells were incubated in 5 mM d-glucose serum-free DMEM in the absence or presence of albumin (0, 30, or 60 μg/ml) with or without tiron (10−4 M) for 16 hours. (A) Caspase-3 activity assay. (B) Immunoblotting for PARP expression and cleavage in HK-2 cells in 5 mM d-glucose plus 20 mM d-mannitol or 25 mM d-glucose in the presence or absence of 60 μg/ml albumin. (C) HK-2 cell apoptosis analyzed by TUNEL staining assay. HK-2 cells were incubated in 5 mM d-glucose plus 20 mM d-mannitol medium in the absence (a) or in the presence (b) of 60 μg/ml albumin or in 25 mM d-glucose medium in the absence (c) or presence (d) of 60 μg/ml albumin. Arrows indicate the TUNEL-positive cells. (D) Quantification of TUNEL-positive cells in C. Data are means ± SD; n = 3. ***P < 0.005. Magnification, ×200 in C.