Figure 5.—

Glucose limitation-induced unipolar budding pattern is independent of Ste12 and Flo8. Wild type (WT; JCY100), flo8Δ (RCY104), ste12Δ (JCY600), and dig1Δ dig2Δ (JCY501) cells were examined by microscopy. To facilitate assessment of budding patterns, cell walls and cell division junctions were stained with calcofluor white (0.1 g/liter) just before visualization. YPD: cultures were grown to exponential phase in YPD. YP⁻: overnight YPD cultures were diluted 10× directly into YP and incubated for 16 hr.