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. 2010 Jul 5;207(7):1485–1500. doi: 10.1084/jem.20092695

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© 2010 Swanson et al.

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Figure 4. — The TI-2 IgG2c response induced by poly(I:C) is not mediated by B cell–intrinsic TLR3 signaling. (A) NP-specific IgG2c responses in wild type, Tlr3^−/−, Mda5^−/−, and Tlr3^−/− Mda5^−/− mice measured from sera collected preimmunization (day 0) and at 7 d after NP-Ficoll + poly(I:C) immunization. Symbols represent individual mice, and horizontal bars indicate geometric mean. Shown is one representative of two independent experiments performed, with four to five mice per group. *, P < 0.05. (B) NP-specific IgG2c/a antibody responses in mixed bone marrow chimeras 7 d after immunization with NP-Ficoll + poly(I:C). A mixture of bone marrow cells from B6.C20 (Igh^a) and Tlr3^−/− (Igh^b; experimental chimeras; filled) or B6.C20 and wild-type C57BL/6 (Igh^b; control chimeras; open) was adoptively transferred to lethally irradiated B6.C20 hosts. 8 wk after reconstitution, chimeras were immunized and NP-specific antibody measured in preimmune and day-7 sera. Reconstitution frequencies of IgM^a- and IgM^b-expressing B cells were measured as described in the Materials and methods and found to be equivalent between both experimental and control chimeras. Symbols represent individual chimeric mice, and horizontal bars indicate geometric mean. Data were combined from two independent experiments with five to six mice per group. n.d., not detected.