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. 2010 Jul 5;207(7):1485–1500. doi: 10.1084/jem.20092695

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© 2010 Swanson et al.

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Figure 6. — IFN-α induces BCR-stimulated FO B cells to up-regulate CD69 to a greater extent than MZ B cells. (A) Histograms represent flow cytometric analysis of IFNαR1 surface expression on CD21^int CD23^high FO B cells and CD21^high CD23^low MZ B cells isolated from naive wild-type mice. Isotype control antibody staining for both FO (shaded light gray) and MZ (dashed line) B cells is provided. Data are representative from two independent experiments. (B–D) FO and MZ B cells were sorted to ≥94% purity from naive C57BL/6 mice and separately incubated with the indicated stimuli in vitro for 6 or 10 h. After stimulation, surface expression of CD69 (6 h) or CD86 (10 h) was subsequently assessed in both B cell populations by flow cytometry and data depicted as histograms. The following stimuli and doses were used: (B) rIFN-α at 1, 10, or 100 U/ml; (C) rIFN-α at 1 U/ml and anti-IgM F(ab′)₂ at 10 µg/ml; (D) LPS at 10 µg/ml. Med, medium. Data are representative from at least two independent experiments.