Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jul 5;207(7):1465–1474. doi: 10.1084/jem.20091975

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — PbeIK2 leads to translational repression in salivary gland sporozoites. (A) 5 × 10⁵ sporozoites of wild-type P. berghei were dissected from mosquito midguts, hemocoels, and salivary glands. Levels of PbeIF2α-P and total PbeIF2α were revealed by densitometry analysis of immunoblots. Values are shown below the bands. (B) PbeIF2α phosphorylation levels of wild-type and PbeIK2 (-) salivary gland sporozoites were assayed by immunoblots. (C) The same number (5 × 10⁵) of wild-type and PbeIK2 (-) salivary gland sporozoites were exposed to ³⁵S-Met/Cys at 37°C for 30 min. Samples were then resolved on SDS-PAGE for autoradiography. (D) Effect of temperature on the kinetic incorporation of ³⁵S- Met/Cys by wild-type and PbeIK2 (-) salivary gland sporozoites. These data were obtained in two independent experiments.