Fig. 4.

Verification of illumina microarray data using RT-qPCR. RT-qPCR analysis of FOLR3, CDH6, KRT8, Cyclin D2, NPPB, CXCL12, CRABP2 and IGF2 were performed using HAF stem cells from the 1^st, 4^th, 6^th, and 10^th passages. GAPDH served as an internal control and was used for the normalization of each gene in qPCR. For relative mRNA expression, the value for the control group was defined as "0.01" for FOLR3 and "0.1" for CXCL12 and IGF2. For the expression of other genes, the value for the control group was defined as "1.0".